Description
Principle of the assay: The f-PSA kit is a solid phase direct sandwich ELISA method. The unknowns and diluted anti-f-PSAHRP conjugate are added to the wells coated with MAb to f-PSA. The f-PSA molecules present in the standard solution or sera are "sandwiched" between the two antibodies. Following the formation of the coated antibody-antigen-antibody-enzyme complex, the unbound protein and HRP-conjugate are washed off by wash buffer. Upon the addition of the substrate, the intensity of color is proportional to the concentration of f-PSA in the unknowns. A calibration curve is prepared relating color intensity to the concentration of the f-PSA